U.S. Department of the Interior, Drug Program Staff

DOT Urine Specimen Collection Training Test Questions

1. The 49CFR Part 40, "Procedures for Transportation Workplace Drug and Alcohol Testing Programs; Final Rule (2001) is or will be effective on:

A. January 19, 2001
B. April 1, 2001
C. July 30, 2001
D. August 1, 2001

2. A DOT drug test collection site must have:

A. Necessary Personnel, Supervision to provide for the collection
B. Temporary storage, materials, equipment
C. Suitable clean writing surface, facilities for urination
D. A & B
E. All of the above.

3. Which is the preferred type of facility for urination at the collection site?

A. Multi stall restroom with partial-length privacy door
B. Single-toilet room with full-length privacy door
C. None of the Above

4. T/F Collections can be done at medical facilities or in mobile facilities if they meet the requirements of Para 40.41.

5. T/F Collectors are limited to conducting a collection for only one employee at a time.

6. To protect the security and integrity of the urine collection within the collection site, what step does NOT apply under DOT?

A. Securing water sources
B. Ensure no wallets are with employee
C. Blue toilet and tank water
D. Remove possible adulterants
E. Secure possible sources of contaminants

7. Which of the following is NOT a "fatal flaw" during the laboratory inspection?

A. Collector's signature is omitted on certification statement on the CCF
B. Specimen bottle seal is broken or shows evidence of tampering and the split specimen cannot be redesignated
C. Collector's printed name and signature are omitted from the CCF
D. Specimen ID number on specimen bottle and CCF do not match

8. When the specimen temperature was not checked and the "Remarks" line did not contain an entry regarding the temperature being outside of range, the error is called a:

A. Fatal flaw
B. Collector flaw
C. CCF flaw
D. Correctable flaw

9. The ingestion of poppy seed bagels could give a positive urinalysis drug for:

A. THC
B. Cocaine
C. PCP
D. Opiates
E. Amphetamines

10. Validity Testing assays the specimen for:

A. Creatinine
B. pH
C. Specific gravity
D. Adulteration
E. All of the above

11. Under what circumstances would the collector discard an insufficient specimen and urge the employee to drink up to 40 oz of fluid distributed over three hours.

A. Shy Bladder procedure
B. Validity test error
C. Fatal flaw inspection
D. Chain of custody problem

12. As an employee, you have refused to take a drug test if you:

A. Fail to appear for a drug test after notification by employer
B. Fail to remain at the test site until the testing process is completed
C. Fail to provide a urine specimen
D. Fail to cooperate with any part of the testing process
E. (A) and (C) only
F. A through D

13. The collector must send the copy 2 of the CCF to the MRO and copy 4 to the DER if the three hour period is completed during shy bladder procedures by what method and within what time period?

A. E-mail within 2 hours
B. Express mail within 8 hours
C. Facsimile within 24 hours or next business day
D. None of the above

14. T/F If during or shortly after the collection process, the collector becomes aware of any event that prevents the completion of a valid test of collection, the collector may conduct another collection.

15. T/F During a collection if the employee asks, the collector must provide an ID with their name.

16. The New CCF effective August 1, 2001 has how many pages?

A. 7
B. 3
C. 6
D. 5

17. T/F The CCF must include the names, addresses, telephone numbers and fax numbers of the employer and the MRO.

18. T/F As the collector, you may use a CCF with your name, address, telephone number and fax number pre-printed, but under no circumstances may you sign the form before collection.

19. T/F The only employee information that is provided to the lab is the employee's social security number or employee ID.

20. T/F The use of a non-DOT chain of custody form is a correctable flaw.

21. What steps must operators of collection sites take before each collection to protect the security and integrity of urine collections?

A. Secure any water sources or otherwise make them unavailable to employees (e.g., turn off water inlet, tape handles to prevent opening faucets); ensure that the water in the toilet is blue;
B. Ensure that no soap, disinfectants, cleaning agents, or other possible adulterants are present; inspect the site to ensure that no foreign or unauthorized substances are present; tape or otherwise secure shut any movable toilet tank top, or put bluing in the tank;
C. Ensure that undetected access (e.g., through a door not in your view) is not possible; secure areas and items (e.g., ledges, trash receptacles, paper towel holders, under-sink areas) that appear suitable for concealing contaminants; and post "Limited Access" signs if required to restrict entry and provide privacy.
D. All of the above.

22. As a collector, you must take the following additional steps to ensure security during the collection process:

A. To avoid distraction that could compromise security, you are limited to conducting a collection for only one employee at a time.
B. To the greatest extent you can, keep an employee's collection container within view of both you and the employee between the time the employee has urinated and the specimen is sealed.
C. Ensure you are the only person in addition to the employee who handles the specimen before it is poured into the bottles and sealed with tamper-evident seals.
D. In the time between when the employee gives you the specimen and when you seal the specimen, remain within the collection site.
E. Maintain personal control over each specimen and CCF throughout the collection process.
F. All of the above.

23. The collector must check for the following when the employee presents a specimen.

A. Temperature, pH, signs of tampering
B. Temperature, signs of tampering, creatinine
C. Sufficiency of specimen. Temperature, specific gravity
D. Sufficiency of specimen, temperature, signs of tampering

24. T/F The employee will be made to remove outer clothing (e.g., coveralls, jacket, coat, hat) and to leave briefcases, purses, or other belongings with you or in a mutually agreeable location and to show the collector the contents of their pockets prior to collection.

25. T/F The employee must not list medications they are currently taking on the CCF.

26. Conduct by the employee that clearly indicates an attempt to tamper with the specimen must be reported:

A. To the DER
B. By writing in the "Remarks" line of the CCF (Step 2)
C. Collection site supervisor
D. All of the above

27. T/F The acceptable temperature range for a specimen is 32-38 degrees C/90-100 degrees F.

28. The temperature must be checked within how many minutes of the employee presenting the specimen.

A. 5
B. 3
C. 10
D. 4

29. T/F When the collector has two sets of specimens from on employee as in a case where a second specimen is collected under direct observation because the temperature is out of range, the collector must process both sets to the laboratory.

30. Excessive foaming when shaken, blue dye in specimen, unusual odor, and/or foreign objects in the specimen indicates:

A. Signs of malnutrition
B. Signs of tampering
C. Prescription medication
D. Specific gravity test results

31. T/F In direct observation, the observer watches the urine go from the employee's body into the container, but the observer must not handle the specimen.

32. T/F Observers and monitors must be the same sex as the employee.

33. DOT specimen collections are split into two specimen bottles by what volumes;

A. 10ml Primary/20ml secondary
B. 15ml Primary/10ml secondary
C. 25ml Primary/15ml secondary
D. 30ml Primary/15ml secondary

34. T/F Under the observation of the collector, the employee will pour the specimen from the collection cup into the specimen bottles, cap the bottles and place the tamper-evident seals over the specimen bottle caps.

35. The "Remarks" in Step 2 of the CCF is used to document:

A. Temperature out of range
B. Monitor's or Observer's name
C. Shy bladder cases
D. Refusal to initial the tamper-evident tape or CCF copy 2 Step 5.
E. C and D
F. A through D

36. T/F In the CCF effective August 1, 2001, the copies are distributed as follows; Copies 1 to the laboratory with the specimen, Copy 2 to the MRO, Copy 4 to the employer, Copy 5 to the employee and Copy 3 to the collector.

37. T/F If the employee refuses to sign the CCF Copy 2 Step 5 and/or initial the tamper-evident tape, the collector prints the employee's name, notes the refusal and processes the specimen.

38. Regarding collector qualification training and proficiency demonstration, if you became a collector before August 1, 2001 and meet the requirements:

A. You must requalify before January 31, 2003
B. You must take a refresher course prior to January 31, 2003
C. You must take error correction training
D. You do not have to meet them again

39. T/F In the time between when the employee gives you the specimen and when you seal the specimen, you and the employee can leave the collection site.

40. A collector places what items in the appropriate pouches of the plastic bag and then seals the bag.

A. CCF Copy1 and CCF Copy 3
B. CCF Copy 2 and specimen
C. Specimen only
D. Specimen and Copy 1
E. None of the above

41. DOT specimens can only be tested for:

A. Marijuana metabolites(THC), cocaine metabolites (COC), anabolic steroids (AST), Opiate metabolites (OPI), phencyclidine (PCP)
B. Marijuana metabolites(THC), cocaine metabolites (COC), amphetamines (AMP), Opiate metabolites (OPI), phencyclidine (PCP)
C. Marijuana metabolites(THC), barbiturate (BAR), amphetamines (AMP), Opiate metabolites (OPI), phencyclidine (PCP)
D. Marijuana metabolites(THC), cocaine metabolites (CAC), amphetamines (AMP), Opiate metabolites (OPUSES), albuterol sulfates (ABS)

42. T/F The laboratory retains a specimen that was reported positive, adulterated, substituted, or invalid results for a minimum of one year.

43. In addition to the periodic inspections, what other methods are used to assure quality control of the analysis process.

A. Blind samples
B. Internal quality control samples
C. A and B
D. None of the above

44. T/F The plastic specimen bottles must have markings clearly indicating 30 ml and 15 ml.

45. The absorbent material in a collection kit must be able to absorb at least:

A. 100 ml
B. 45 ml
C. 30ml
D. 15 ml

46. T/F The employee's current medication is entered in the "Remarks" of the MRO CCF Copy 2.

47. Service Agents would most likely be used for:

A. Employee relations
B. MRO
C. Urine and Alcohol on site collectors
D. B and C only
E. All of the above

48. T/F A collector is allowed to combine urine collected from two separate voids to create 45 ml if each void was an insufficient quantity.

49. Which of the following is an appropriate reason for not providing a specimen.

A. Not enough sleep
B. Against donor religion
C. Not in the mood
D. None of the above

50. The collector may ask the donor which of the following questions.

A. Where they went to high school
B. What their favorite color is
C. What their favorite animal is
D. A and C only
E. None of the above

51. Collectors should be considered to be which of the following

A. Familiar with 49 CFR Part 40
B. Knuckleheads
C. Rocket Scientists
D. Any of the above

DOT Urine Specimen Collection Training Test Questions and Answers

1. The 49CFR Part 40, "Procedures for Transportation Workplace Drug and Alcohol Testing Programs; Final Rule (2001) is or will be effective on:

A. January 19, 2001
B. April 1, 2001
C. July 30, 2001
D. August 1, 2001

Ref: 40.3 Ans: D

2. A DOT drug test collection site must have:

A. Necessary Personnel, Supervision to provide for the collection
B. Temporary storage, materials, equipment
C. Suitable clean writing surface, facilities for urination
D. A & B
E. All of the above

Ref: 40.41 Ans E

3. Which is the preferred type of facility for urination at the collection site?

A. Multi stall restroom with partial-length privacy door
B. Single-toilet room with full-length privacy door
C. None of the Above

Ref: 40.41e Ans B

4. T/F Collections can be done at medical facilities or in mobile facilities if they meet the requirements of Para 40.41.

Ref 40.41g Ans True

5. T/F Collectors are limited to conducting a collection for only one employee at a time.

Ref: 40.43d1 Ans True

6. To protect the security and integrity of the urine collection within the collection site, what step does NOT apply under DOT?

A. Securing water sources
B. Ensure no wallets are with employee
C. Blue toilet and tank water
D. Remove possible adulterants
E. Secure possible sources of contaminants

Ref: 40.83c Ans B

7. Which of the following is NOT a "fatal flaw" during the laboratory inspection?

Ref: 40.83c Ans: A

8. When the specimen temperature was not checked and the "Remarks" line did not contain an entry regarding the temperature being outside of range, the error is called a:

A. Fatal flaw
B. Collector flaw
C. CCF flaw
D. Correctable flaw

Ref: 40.83e Ans D

9. The ingestion of poppy seed bagels could give a positive urinalysis drug for:

A. THC
B. Cocaine
C. PCP
D. Opiates
E. Amphetamines

Ref: 40.85 Ans D

10. Validity Testing assays the specimen for:

A. Creatinine
B. pH
C. Specific gravity
D. Adulteration
E. All of the above

Ref: 40.91 Ans E

11. Under what circumstances would the collector discard an insufficient specimen and urge the employee to drink up to 40 oz of fluid distributed over three hours.

A. Shy Bladder procedure
B. Validity test error
C. Fatal flaw inspection
D. Chain of custody problem

Ref: 40.193 Ans A

12. As an employee, you have refused to take a drug test if you:

Ref: 40.191 Ans F

13. The collector must send the copy 2 of the CCF to the MRO and copy 4 to the DER if the three hour period is completed during shy bladder procedures by what method and within what time period?

A. E-mail within 2 hours
B. Express mail within 8 hours
C. Facsimile within 24 hours or next business day
D. None of the above

Ref: 40.193a8c Ans C

Ref: 40.205 Ans True

15. T/F During a collection if the employee asks, the collector must provide an ID with their name.

Ref: 40.61 Ans True

16. The New CCF effective August 1, 2001 has how many pages?

A. 7
B. 3
C. 6
D. 5

Ref: 40.45 Ans D

17. T/F The CCF must include the names, addresses, telephone numbers and fax numbers of the employer and the MRO.

Ref: 40.45 Ans True

18. T/F As the collector, you may use a CCF with your name, address, telephone number and fax number pre-printed, but under no circumstances may you sign the form before collection.

Ref: 40.45 Ans True

19. T/F The only employee information that is provided to the lab is the employee's social security number or employee ID.

Ref: 40.45 Ans True

20. T/F The use of a non-DOT chain of custody form is a correctable flaw.

Ref: 40.47 Ans True

21. What steps must operators of collection sites take before each collection to protect the security and integrity of urine collections?

A. Secure any water sources or otherwise make them unavailable to employees (e.g., turn off water inlet, tape handles to prevent opening faucets); ensure that the water in the toilet is blue;
B. Ensure that no soap, disinfectants, cleaning agents, or other possible adulterants are present; inspect the site to ensure that no foreign or unauthorized substances are present; tape or otherwise secure shut any movable toilet tank top, or put bluing in the tank;
C. Ensure that undetected access (e.g., through a door not in your view) is not possible; secure areas and items (e.g., ledges, trash receptacles, paper towel holders, under-sink areas) that appear
suitable for concealing contaminants; and post "Limited Access" signs if required to restrict entry and provide privacy.
D. All of the above.

Ref: 40.61 Ans D

22. As a collector, you must take the following additional steps to ensure security during the collection process:

Ref: 40.65 Ans F

23. The collector must check for the following when the employee presents a specimen.

Ref: 40.65 Ans D

Ref: 40.61 Ans True

25. T/F The employee must not list medications they are currently taking on the CCF.

Ref: 40.61 Ans True

26. Conduct by the employee that clearly indicates an attempt to tamper with the specimen must be reported:

A. To the DER
B. By writing in the "Remarks" line of the CCF (Step 2)
C. Collection site supervisor
D. All of the above

Ref: 40.63 Ans D

27. T/F The acceptable temperature range for a specimen is 32-38 degrees C/90-100 degrees F.

Ref: 40.65 Ans True

28. The temperature must be checked within how many minutes of the employee presenting the specimen.

A. 5
B. 3
C. 10
D. 4

Ref: 40.65 Ans D

Ref: 40.65 Ans True

30. Excessive foaming when shaken, blue dye in specimen, unusual odor, and/or foreign objects in the specimen indicates:

A. Signs of malnutrition
B. Signs of tampering
C. Prescription medication
D. Specific gravity test results

Ref: 40.65 Ans B

31. T/F In direct observation, the observer watches the urine go from the employee's body into the container, but the observer must not handle the specimen.

Ref: 40.67 Ans True

32. T/F Observers and monitors must be the same sex as the employee.

Ref: 40.657/40.69 Ans True

33. DOT specimen collections are split into two specimen bottles by what volumes;

A. 10ml Primary/20ml secondary
B. 15ml Primary/10ml secondary
C. 25ml Primary/15ml secondary
D. 30ml Primary/15ml secondary

Ref: 40.71 Ans D

Ref: 40.71 Ans False

35. The "Remarks" in Step 2 of the CCF is used to document:

A. Temperature out of range
B. Monitor's or Observer's name
C. Shy bladder cases
D. Refusal to initial the tamper-evident tape or CCF copy 2 Step 5
E. C and D
F. A through D

Ref: Subpart E Ans F

Ref: 40.73 Ans True

37. T/F If the employee refuses to sign the CCF Copy 2 Step 5 and/or initial the tamper-evident tape, the collector prints the employee's name, notes the refusal and processes the specimen.

Ref: 40.73 Ans True

38. Regarding collector qualification training and proficiency demonstration, if you became a collector before August 1, 2001 and meet the requirements:

A. You must requalify before January 31, 2003
B. You must take a refresher course prior to January 31, 2003
C. You must take error correction training
D. You do not have to meet them again, however, you will need refresher training every 5 years.

Ref: 40.33 Ans D

39. T/F In the time between when the employee gives you the specimen and when you seal the specimen, you and the employee can leave the collection site.

Ref: 40.43 Ans False

40. A collector places what items in the appropriate pouches of the plastic bag and then seals the bag.

A. CCF Copy1 and CCF Copy 3
B. CCF Copy 2 and specimen
C. Specimen only
D. Specimen and Copy 1
E. None of the above

Ref: 40.73 Ans D

41. DOT specimens can only be tested for:

Ref: 40.85 Ans B

42. T/F The laboratory retains a specimen that was reported positive, adulterated, substituted, or invalid results for a minimum of one year.

Ref: 40.99 Ans True

43. In addition to the periodic inspections, what other methods are used to assure quality control of the analysis process.

A. Blind samples
B. Internal quality control samples
C. A and B
D. None of the above

Ref: 40.103 Ans C

44. T/F The plastic specimen bottles must have markings clearly indicating 30 ml and 15 ml.

Ref: Appendix A Ans True

45. The absorbent material in a collection kit must be able to absorb at least:

A. 100 ml
B. 45 ml
C. 30ml
D. 15 ml

Ref: Appendix A Ans B

46. T/F The employee's current medication is entered in the "Remarks" of the MRO CCF Copy 2.

Ref: 40.61f5g Ans False

47. Service Agents would most likely be used for:

A. Employee relations
B. MRO
C. Urine and Alcohol on site collectors
D. B and C only
E. All of the above

Ref: 40.3 Ans D

48. T/F A collector is allowed to combine urine collected from two separate voids to create 45 ml if each void was an insufficient quantity.

Ref: 40.65 Ans False

49. Which of the following is an appropriate reason for not providing a specimen.

A. Not enough sleep
B. Against donor religion
C. Not in the mood
D. None of the above

Ans D

50. The collector may ask the donor which of the following questions.

A. Where they went to high school
B. What their favorite color is
C. What their favorite animal is
D. A and C only
E. None of the above

Ans E

51. Collectors should be considered to be which of the following

A. Familiar with 49 CFR Part 40
B. Knuckleheads
C. Rocket Scientists
D. Any of the above

Ans D

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